Package 8 – peptide synthesized by SICGEN
Description
• Immunization using 1 goat • Pre-immune serum (5-10 ml) • 5 immunizations (1, 3, 5, 7, 9th week) • 4 serum bleeding (5, 7, 9, 12th week) • 500-600 ml antiserum per animal (or 500-1000 ml more *) • ELISA test (until titer reaches 20,000) • Optional Western blotting test available • Serum separation • 12 weeks protocol period |
No of Animals 1 |
Protein/peptide Required
• SICGEN synthesizes 20 mg peptide (up to 20 aa, purity : >70%)** • Customer provide antigen information (peptide sequence, protein name, GenBank Acc n.) |
Pre-immune serum Yes |
Conjugation included Yes |
ELISA testing ELISA test (until titer reaches >20,000) |
Western Blot Optional |
Purification Optional
• Protein G purification • Antigen-affinity purification |
Antibody to Customer 500-600 ml antiserum per animal* |
Notes *If the customer decides not to continue the project, extra 500-1000 ml of serum will be
obtained by terminal bleed; this option might not be available ** If antigen-affinity purification is performed, at least 10 mg of peptide will required |