Package 8 – peptide synthesized by SICGEN

Description

•    Immunization using 1 goat

•    Pre-immune serum (5-10 ml)

•    5 immunizations (1, 3, 5, 7, 9th week)

•    4 serum bleeding (5, 7, 9, 12th week)

•    500-600 ml antiserum per animal (or 500-1000 ml more *)

•    ELISA test (until titer reaches 20,000)

•    Optional Western blotting test available

•    Serum separation

•    12 weeks protocol period

No of Animals 1
Protein/peptide Required

•    SICGEN synthesizes 20 mg peptide (up to 20 aa, purity : >70%)**

•    Customer provide antigen information (peptide sequence, protein name, GenBank Acc n.)

Pre-immune serum Yes
Conjugation included Yes
ELISA testing ELISA test (until titer reaches >20,000)
Western Blot Optional
Purification Optional

•    Protein G purification

•    Antigen-affinity purification

Antibody to Customer 500-600 ml antiserum per animal*
Notes *If the customer decides not to continue the project, extra 500-1000 ml of serum will be

obtained by terminal bleed; this option might not be available

** If antigen-affinity purification is performed, at least 10 mg of peptide will required