What quantity of antigen is required for antigen affinity purification?
For purification of 50 ml antiserum, about 2-10 mg of protein or 3-10 mg of lyophilized peptide are required to couple to the affinity matrix column. The yield of antibody produced can range from 5 to 10 % of total immunoglobulins and is based on quality of immune response. Generally, a minimum of 10 mg of antibody can be obtained. If smaller amounts are available, less quantity can be used for purification. Please inquire SICGEN. The protein should not contain glycine or Tris buffers.