Package 4 – peptide synthesized by SICGEN
| Description
• Immunization using 1 goat • Pre-immune serum (5-10 ml) • 3 immunizations (1, 2, 3rd week) • 1 serum bleeding (4th week) • 200-250 ml antiserum per animal (or 500-1000 ml*) • ELISA test (4th week) • Optional Western blotting test available • Serum separation • 4 weeks protocol period |
| No of Animals 1 |
| Protein/peptide Required
• SICGEN synthesizes 20 mg peptide (up to 20 aa, purity : >70%)** • Antigen information (peptide sequence, GenBank Acc n., etc.) |
| Pre-immune serum Yes |
| Conjugation included Yes |
| ELISA testing 4th week |
| Western Blot Optional |
| Purification Optional
• Protein G purification • Antigen-affinity purification |
| Antibody to Customer 200-250 ml antiserum per animal* |
| Notes * If the customer decides not to continue the project, extra 500-1000 ml of serum will be obtained by
terminal bleed; this option might not be available **If antigen-affinity purification is performed, at least 10 mg of peptide will required |
