Package 2 – protein synthesized by SICGEN
Description
• Immunization using 1 goat • Pre-immune serum (5-10 ml) • 3 immunizations (1, 2, 3rd week) • 1 serum bleeding (5th week) • 200-250 ml antiserum per animal (or 500-1000 ml*) • ELISA test (4th week) • Optional Western blotting test available • Serum separation • 4 weeks protocol period |
No of Animals 1 |
Protein/peptide Required
• SICGEN synthesizes 2-10 mg protein (bacterial expression), 1-2 mg are used for antibody production, 0.1 mg for ELISA and at least 1-2 mg used for epitope affinity purification or delivered to researcher • Customer provide antigen information (protein name, sequence and GenBank Acc n.) |
Pre-immune serum Yes |
Conjugation included Yes |
ELISA testing 4th week |
Western Blot Optional |
Purification Optional
• Protein G purification • Antigen-affinity purification |
Antibody to Customer 200-250 ml antiserum per animal* |
Notes * If the customer decides not to continue the project, extra 500-1000 ml of serum will be obtained by
terminal bleed; this option might not be available |